Human FGF21 protein, His Tag | PRP100221

Human FGF21 protein, His Tag | PRP100221 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from mature form of human FGF21 (NP_061986.1) (His 29-Ser 209) was expressed, with a polyhistide tag at the N-terminus.

Activity: Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1­1 µg/mL in the presence of 2.5 µg/mL of rmKlotho β.

protein Lenght: The recombinant human FGF21 consisting of 188 amino acids and has a calculated molecular mass of 20.2 kDa. It migrates as an 23 kDa band in SDS-PAGE under reducing conditions as predicted.

Preparation: E. coli

Purity: > 95 % as determined by SDS-PAGE

Formulation: Lyophilized from 50 mM Tris, 10 % glycerol, 0.05 % Brig 35, pH 8.0

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 20.2 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human FGF21 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human FGF21 protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.58% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 has a hydrophobic amino terminus, which is a typical signal sequence, and appears to be a secreted protein. The metabolic regulator fibroblast growth factor 21 (FGF21) has antidiabetic properties in animal models of diabetes and obesity. FGF21 is a novel adipokine associated with obesity-related metabolic complications in humans. The paradoxical increase of serum FGF21 in obese individuals, which may be explained by a compensatory response or resistance to FGF21, warrants further investigation. FGF-21, which we have identified as a novel metabolic factor, exhibits the therapeutic characteristics necessary for an effective treatment of diabetes.

Alternative Names: UNQ3115 / PRO10196; Fibroblast growth factor 21; FGF21

Search Names: UNQ3115 / PRO10196; Fibroblast growth factor 21; FGF21

Tag: FGF21