Human FGF5 (Fibroblast Growth Factor 5) ELISA Kit | ELK3508

Human FGF5 (Fibroblast Growth Factor 5) ELISA Kit | ELK3508 | Gentaur UK, US & Europe Distribution

Alternative Names: HBGF5; Smag82; Heparin-binding growth factor 5

Species: Human

Assay Type: Sandwich

Sensitivity: 4.99 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 5 (FGF5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor 5 (FGF5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 5 (FGF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 5 (FGF5) in the samples is then determined by comparing the OD of the samples to the standard curve.