Human Fibroleukin (FGL2) ELISA Kit | KTE62664

Human Fibroleukin (FGL2) ELISA Kit | KTE62664 | Gentaur UK, US & Europe Distribution

Application: This Human Fibroleukin (FGL2) ELISA Kit employs a two-site sandwich ELISA to quantitate FGL2 in samples. An antibody specific for FGL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Fibroleukin microplate
• Human Fibroleukin standard
• Human Fibroleukin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Fibroleukin (FGL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human FGL2. No significant cross-reactivity or interference between Human FGL2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: FGL2 is a 439 amino acid secreted protein that is similar to the β- and γ-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED) . The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds.This protein may play a role in physiologic functions at mucosal sites.Fgl2 is a protein that exhibits pleiotropic effects within the body and is an important immune regulator of both innate and adaptive responses. The protein exists as both a type II transmembrane protein found on the surface of macrophages and endothelial cells and can be constitutively secreted by both CD4+ and CD8+ T cells.

Alternative Names: FGL2; T49; pT49; fibrinogen-like protein 2; fibroleukin

Search name: FGL2; T49; pT49; fibrinogen-like protein 2; fibroleukin

Tag: FGL2