Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA Kit | AE58737HU

Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA Kit | AE58737HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: FNDC5

Alternative Name: FRCP2; Irisin; Fibronectin type III repeat-containing protein 2

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 6.9 pg/mL

Intra-Assay: ≤6.5%

Inter-Assay: ≤9.1%

Recovery: 1, 03

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate FNDC5 in samples. An antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: FNDC5 contains an N-terminal signal peptide, a fibronectin type III domain, and a transmembrane domain. The 2 proteins share 60% identity in the FNIII domain and 86% in the transmembrane domain. Northern blot analysis of multiple mouse tissues detected highest expression of Fndc5 in adult brain and heart, and low expression in lung, skeletal muscle, and testis. Northern blot analysis of mouse embryonic mRNA first detected Fndc5 expression at embryonic day 11, with strongest expression at E15 and decreasing expression at E17. In situ hybridization demonstrated expression of Fndc5 in the fore- and midbrain, as well as in the brachial arches and the heart. Overexpression of a GFP-Fndc5 fusion protein in COS cells showed localization predominantly in the endoplasmic reticulum, with small amounts in the plasma membrane.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .