Human FKBP4 (FK506 Binding Protein 4) ELISA Kit | ELK4738

Human FKBP4 (FK506 Binding Protein 4) ELISA Kit | ELK4738 | Gentaur UK, US & Europe Distribution

Alternative Names: FKBP52; FKBP59; HBI; Hsp56; p52; Rotamase; 51 kDa FK506-binding protein; 59 kDa immunophilin; HSP-binding immunophilin; Peptidyl-prolyl cis-trans isomerase FKBP4

Species: Human

Assay Type: Sandwich

Sensitivity: 13.2 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 4 (FKBP4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FK506 Binding Protein 4 (FKBP4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 4 (FKBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 4 (FKBP4) in the samples is then determined by comparing the OD of the samples to the standard curve.