Human FLII (Flightless I Homolog) ELISA Kit | ELK3650

Human FLII (Flightless I Homolog) ELISA Kit | ELK3650 | Gentaur UK, US & Europe Distribution

Alternative Names: FLI; FLIL; Fli1

Species: Human

Assay Type: Sandwich

Sensitivity: 29.2 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flightless I Homolog (FLII) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Flightless I Homolog (FLII) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flightless I Homolog (FLII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Flightless I Homolog (FLII) in the samples is then determined by comparing the OD of the samples to the standard curve.