Human FLNa (Filamin A Alpha) ELISA Kit | ELK4516

Human FLNa (Filamin A Alpha) ELISA Kit | ELK4516 | Gentaur UK, US & Europe Distribution

Alternative Names: ABP280; FLN-A; ABPX; FLN; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; Actin Binding Protein 280; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.65 ng/mL

Standard: 120 ng/mL

Detection Range: 1.88-120 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha (FLNa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Filamin A Alpha (FLNa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin A Alpha (FLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin A Alpha (FLNa) in the samples is then determined by comparing the OD of the samples to the standard curve.