Human Forkhead box O3 (FOXO3) ELISA Kit | KTE62253

Human Forkhead box O3 (FOXO3) ELISA Kit | KTE62253 | Gentaur UK, US & Europe Distribution

Application: This Human Forkhead box O3 (FOXO3) ELISA Kit employs a two-site sandwich ELISA to quantitate FOXO3 in samples. An antibody specific for FOXO3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFOXO3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOXO3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOXO3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Forkhead box O3 microplate
• Human Forkhead box O3 standard
• Human Forkhead box O3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Forkhead box O3 (FOXO3) ELISA Kit has high sensitivity and excellent specificity for detection of Human FOXO3. No significant cross-reactivity or interference between Human FOXO3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: FOXO3 belongs to the O subclass of the forkhead family of transcription factors which are characterized by a distinct fork head DNA binding domain. There are three other FoxO family members in humans, FOXO1, FOXO4 and FOXO6. These transcription factors share the ability to be inhibited and translocated out of the nucleus (aside from FOXO6, which may be constitutively nuclear) on phosphorylation by proteins such as Akt/PKB in the PI3K signalling pathway. Other post-translational modifications including acetylation and methylation are seen and can result in increased or altered FOXO3a activity. This protein likely functions as a trigger for apoptosis through upregulation of genes necessary for cell death, such as Bim and PUMA, or downregulation of anti-apoptotic proteins such as FLIP.

Alternative Names: FOXO3; AF6q21; DKFZp781A0677; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; MGC12739; MGC31925; forkhead box O3A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead; Drosophila; homolog of; in rhabdomyosarcoma-like 1

Search name: FOXO3; AF6q21; DKFZp781A0677; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; MGC12739; MGC31925; forkhead box O3A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead; Drosophila; homolog of; in rhabdomyosarcoma-like 1

Tag: FOXO3