Human FOXP3 (Forkhead Box Protein P3) ELISA Kit | ELK2241

Human FOXP3 (Forkhead Box Protein P3) ELISA Kit | ELK2241 | Gentaur UK, US & Europe Distribution

Alternative Names: FOX-P3; AIID; DIETER; IPEX; JM2; PIDX; XPID; SCURFIN; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked

Species: Human

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Apoptosis;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein P3 (FOXP3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Forkhead Box Protein P3 (FOXP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein P3 (FOXP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein P3 (FOXP3) in the samples is then determined by comparing the OD of the samples to the standard curve.