Human FPN (Ferroportin) ELISA Kit | ELK5475

Human FPN (Ferroportin) ELISA Kit | ELK5475 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC40A1; FPN1; HFE4; IREG1; MTP1; SLC11A3; Solute Carrier Family 40, Member 1; Iron-Regulated Transporter

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 91.4 pg/mL

Standard: 20000 pg/mL

Detection Range: 312.5-20000 pg/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Infection immunity;Hematology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ferroportin (FPN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ferroportin (FPN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferroportin (FPN) in the samples is then determined by comparing the OD of the samples to the standard curve.