Human FSH (Follicle Stimulating Hormone) ELISA Kit | ELK1221

Human FSH (Follicle Stimulating Hormone) ELISA Kit | ELK1221 | Gentaur UK, US & Europe Distribution

Alternative Names: FSH-B; FSH-beta

Species: Human

Assay Type: Sandwich

Sensitivity: 0.34 mIU/mL

Standard: 50 mIU/mL

Detection Range: 0.79-50 mIU/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology;Reproductive science;Genetic science;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follicle Stimulating Hormone (FSH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Follicle Stimulating Hormone (FSH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follicle Stimulating Hormone (FSH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follicle Stimulating Hormone (FSH) in the samples is then determined by comparing the OD of the samples to the standard curve.