Human FUT3 (Fucosyltransferase 3) ELISA Kit | ELK4024

Human FUT3 (Fucosyltransferase 3) ELISA Kit | ELK4024 | Gentaur UK, US & Europe Distribution

Alternative Names: CD174; FUT3; FT3B; LE; Les; FucT-III; Lewis FT; Galactoside 3 (4) -L-Fucosyltransferase, Lewis Blood Group; Blood group Lewis alpha-4-fucosyltransferase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosyltransferase 3 (FUT3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fucosyltransferase 3 (FUT3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosyltransferase 3 (FUT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fucosyltransferase 3 (FUT3) in the samples is then determined by comparing the OD of the samples to the standard curve.