Human GA (Glycated Albumin) ELISA Kit | ELK8432

Human GA (Glycated Albumin) ELISA Kit | ELK8432 | Gentaur UK, US & Europe Distribution

Alternative Names: Glycated Albumin, GA

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 5.16 pmol/mL

Standard: 1000 pmol/mL

Detection Range: 15.63-1000 pmol/mL

Sample Type: serum, plasma

Assay Lenght: 2h

Research Area: diabetic nephropathy

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycated Albumin (GA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycated Albumin (GA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycated Albumin (GA) in the samples is then determined by comparing the OD of the samples to the standard curve.