Human GAL2 (Galectin 2) ELISA Kit | ELK2891

Human GAL2 (Galectin 2) ELISA Kit | ELK2891 | Gentaur UK, US & Europe Distribution

Alternative Names: LGALS2; HL14; Lectin, Galactoside-Binding Soluble 2; Beta-galactoside-binding lectin L-14-II; Lactose-binding lectin 2; S-Lac lectin 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.128 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 2 (GAL2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Galectin 2 (GAL2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 2 (GAL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galectin 2 (GAL2) in the samples is then determined by comparing the OD of the samples to the standard curve.