Human GARS (Glycyl tRNA Synthetase) ELISA Kit | ELK3960

Human GARS (Glycyl tRNA Synthetase) ELISA Kit | ELK3960 | Gentaur UK, US & Europe Distribution

Alternative Names: CMT2D; CMT2-D; DSMAV; GlyRS; SMAD1; EJ; Charcot-Marie-Tooth Neuropathy 2D; Glycine tRNA Ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase

Species: Human

Assay Type: Sandwich

Sensitivity: 6.7 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycyl tRNA Synthetase (GARS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycyl tRNA Synthetase (GARS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycyl tRNA Synthetase (GARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycyl tRNA Synthetase (GARS) in the samples is then determined by comparing the OD of the samples to the standard curve.