Human GCK (Glucokinase) ELISA Kit | ELK1111

Human GCK (Glucokinase) ELISA Kit | ELK1111 | Gentaur UK, US & Europe Distribution

Alternative Names: GK; GLK; HHF3; HK4; HKIV; HXKP; MODY2; Hexokinase-D; Hexokinase 4; Maturity Onset Diabetes Of The Young 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.23 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucokinase (GCK) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucokinase (GCK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucokinase (GCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucokinase (GCK) in the samples is then determined by comparing the OD of the samples to the standard curve.