Human GF (Glycosylated Ferritin) ELISA Kit | ELK8575

Human GF (Glycosylated Ferritin) ELISA Kit | ELK8575 | Gentaur UK, US & Europe Distribution

Alternative Names: Glycosylated Ferritin; GF

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 10.19nmol/ml

Standard: 2000nmol/ml

Detection Range: 31.25-2000 nmol/ml

Sample Type: serum, plasma

Assay Lenght: 2h

Research Area: Diabetes

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycosylated Ferritin (GF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycosylated Ferritin (GF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycosylated Ferritin (GF) in the samples is then determined by comparing the OD of the samples to the standard curve.