Human GLUT1 (Glucose Transporter 1) ELISA Kit | ELK1800

Human GLUT1 (Glucose Transporter 1) ELISA Kit | ELK1800 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC2A1; SLC2-A1; GLUT; Solute Carrier Family 2 Member 1, Facilitated Glucose Transporter; Glucose transporter type 1, erythrocyte/brain; HepG2 glucose transporter

Species: Human

Assay Type: Sandwich

Sensitivity: 0.116 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 1 (GLUT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucose Transporter 1 (GLUT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 1 (GLUT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose Transporter 1 (GLUT1) in the samples is then determined by comparing the OD of the samples to the standard curve.