Human GLUT3 (Glucose Transporter 3) ELISA Kit | ELK2130

Human GLUT3 (Glucose Transporter 3) ELISA Kit | ELK2130 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3; Glucose transporter type 3, brain

Species: Human

Assay Type: Sandwich

Sensitivity: 0.29 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 3 (GLUT3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucose Transporter 3 (GLUT3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 3 (GLUT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose Transporter 3 (GLUT3) in the samples is then determined by comparing the OD of the samples to the standard curve.