Human Glutathione S Transferase Alpha 1 (GSTA1) ELISA Kit | KTE62448

Human Glutathione S Transferase Alpha 1 (GSTA1) ELISA Kit | KTE62448 | Gentaur UK, US & Europe Distribution

Application: This Human Glutathione S Transferase Alpha 1 (GSTA1) ELISA Kit employs a two-site sandwich ELISA to quantitate GSTA1 in samples. An antibody specific for GSTA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGSTA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSTA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSTA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Glutathione S Transferase Alpha 1 microplate
• Human Glutathione S Transferase Alpha 1 standard
• Human Glutathione S Transferase Alpha 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Glutathione S Transferase Alpha 1 (GSTA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human GSTA1. No significant cross-reactivity or interference between Human GSTA1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione.GSTa1 encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation.

Alternative Names: GSTA1; GSTa1; GST2; GSTA1-1; GTH1

Search name: GSTA1; GSTa1; GST2; GSTA1-1; GTH1

Tag: GSTA1