Human GREM1 (Gremlin 1) ELISA Kit | ELK3100

Human GREM1 (Gremlin 1) ELISA Kit | ELK3100 | Gentaur UK, US & Europe Distribution

Alternative Names: PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

Species: Human

Assay Type: Sandwich

Sensitivity: 57 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: serum, plasma, tissue homogenates, urine and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Developmental science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1 (GREM1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gremlin 1 (GREM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gremlin 1 (GREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gremlin 1 (GREM1) in the samples is then determined by comparing the OD of the samples to the standard curve.