Human GZMB (Granzyme B) ELISA Kit | ELK1658

Human GZMB (Granzyme B) ELISA Kit | ELK1658 | Gentaur UK, US & Europe Distribution

Alternative Names: GZM-B; HLP; CTLA1; CCPI; CGL1; CSP-B; CSPB; CTSGL1; SECT; Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1; Fragmentin 2; Cytotoxic Serine Protease B

Species: Human

Assay Type: Sandwich

Sensitivity: 2.7 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Apoptosis;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme B (GZMB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Granzyme B (GZMB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme B (GZMB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granzyme B (GZMB) in the samples is then determined by comparing the OD of the samples to the standard curve.