Human H2B (Histone H2B) ELISA Kit | ELK4882

Human H2B (Histone H2B) ELISA Kit | ELK4882 | Gentaur UK, US & Europe Distribution

Alternative Names: HIST1H2BA; testis; bA317E16.3; H2B; H2B histone family member U; H2B histone family member U testis specific; H2B histone family, member U, (testis specific) ; H2B testis; H2B1A_HUMAN; H2BFU; H2BT; HIST1H2BA; Histone 1, H2ba; Histone cluster 1 H2ba; Histone H2B; Histone H2B testis; Histone H2B type 1 A; Histone H2B type 1-A; Histone H2B type 1A; STBP; Testis specific histone H2B; Testis-specific histone H2B; TSH 2B; TSH2B; TSH2B.1;

Species: Human

Assay Type: Sandwich

Sensitivity: 1.18 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2B (H2B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Histone H2B (H2B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H2B (H2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H2B (H2B) in the samples is then determined by comparing the OD of the samples to the standard curve.