Human HABP1 (Hyaluronan Binding Protein 1) ELISA Kit | ELK1865

Human HABP1 (Hyaluronan Binding Protein 1) ELISA Kit | ELK1865 | Gentaur UK, US & Europe Distribution

Alternative Names: C1QBP; GC1QBP; SF2p32; GC1Q-R; GC1qR; P32; Complement Component 1 Q Subcomponent Binding Protein; Splicing Factor SF2-Associated Protein; ASF/SF2-associated p32

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronan Binding Protein 1 (HABP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyaluronan Binding Protein 1 (HABP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronan Binding Protein 1 (HABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyaluronan Binding Protein 1 (HABP1) in the samples is then determined by comparing the OD of the samples to the standard curve.