Human HBg1 (Hemoglobin Gamma 1) ELISA Kit | ELK4070

Human HBg1 (Hemoglobin Gamma 1) ELISA Kit | ELK4070 | Gentaur UK, US & Europe Distribution

Alternative Names: HB-G1; HBGA; HBGR; HSGGL1; Gamma-1-globin

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 195.5 ng/mL

Standard: 30000 ng/mL

Detection Range: 468.75-30000 ng/mL

Sample Type: Serum, plasma, erythrocyte lysates.

Assay Lenght: 2h

Research Area: Hematology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Gamma 1 (HBg1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin Gamma 1 (HBg1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Gamma 1 (HBg1) in the samples is then determined by comparing the OD of the samples to the standard curve.