Human HBg2 (Hemoglobin Gamma 2) ELISA Kit | ELK4962

Human HBg2 (Hemoglobin Gamma 2) ELISA Kit | ELK4962 | Gentaur UK, US & Europe Distribution

Alternative Names: Gamma-2-globin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.62 μg/mL

Standard: 100 μg/mL

Detection Range: 1.57-100 μg/mL

Sample Type: Serum, plasma, erythrocyte lysates.

Assay Lenght: 3.5h

Research Area: Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin Gamma 2 (HBg2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin Gamma 2 (HBg2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin Gamma 2 (HBg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Gamma 2 (HBg2) in the samples is then determined by comparing the OD of the samples to the standard curve.