Human HCN4 (Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4) ELISA Kit | ELK5355

Human HCN4 (Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4) ELISA Kit | ELK5355 | Gentaur UK, US & Europe Distribution

Alternative Names: HCN4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) in the samples is then determined by comparing the OD of the samples to the standard curve.