Human HE4 (Epididymal protein 4) ELISA kit | ELK8581

Human HE4 (Epididymal protein 4) ELISA kit | ELK8581 | Gentaur UK, US & Europe Distribution

Alternative Names: Epididymis Protein 4; dJ461P17.6 ; EDDM4 ; epididymal protein 4 ; Epididymal secretory protein E4 ; Epididymis specific whey acidic protein type four disulfide core ; HE 4 ; Major epididymis specific protein E4 ; Major epididymis-specific protein E4 ; MGC57529 ; Putative protease inhibitor WAP5 ; WAP 5 ; WAP domain containing protein HE4 ; WAP domain containing protein HE4 V4 ; WAP four disulfide core domain 2 ; WAP four disulfide core domain protein 2 ; WAP four-disulfide core domain protein 2 ; WAP5 ; WFDC 2 ; WFDC2 ; WFDC2_HUMAN

Species: Human

Assay Type: Sandwich

Sensitivity: 5.15 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Ovarian cancer

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epididymal protein 4 (HE4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epididymal protein 4 (HE4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epididymal protein 4 (HE4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epididymal protein 4 (HE4) in the samples is then determined by comparing the OD of the samples to the standard curve.