Human HGFR (Hepatocyte Growth Factor Receptor) ELISA Kit | ELK3712

Human HGFR (Hepatocyte Growth Factor Receptor) ELISA Kit | ELK3712 | Gentaur UK, US & Europe Distribution

Alternative Names: CMET; RCCP2; C-Met; Met Proto-Oncogene; Mesenchymal-Epithelial Transition Factor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; Tyrosine-protein kinase Met

Species: Human

Assay Type: Sandwich

Sensitivity: 49 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor Receptor (HGFR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepatocyte Growth Factor Receptor (HGFR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor Receptor (HGFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Growth Factor Receptor (HGFR) in the samples is then determined by comparing the OD of the samples to the standard curve.