Human Histidyl-tRNA synthetase, cytoplasmic (HARS) ELISA kit | ELK8405

Human Histidyl-tRNA synthetase, cytoplasmic (HARS) ELISA kit | ELK8405 | Gentaur UK, US & Europe Distribution

Alternative Names: FLJ20491, HRS, HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase

Species: Human

Assay Type: Sandwich

Sensitivity: 5.4 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Biosynthesis/Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HARS (Histidyl-tRNA synthetase, cytoplasmic) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HARS (Histidyl-tRNA synthetase, cytoplasmic) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HARS (Histidyl-tRNA synthetase, cytoplasmic), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HARS (Histidyl-tRNA synthetase, cytoplasmic) in the samples is then determined by comparing the OD of the samples to the standard curve.