Human Histone deacetylase-2 (HDAC2) ELISA Kit | KTE62687

Human Histone deacetylase-2 (HDAC2) ELISA Kit | KTE62687 | Gentaur UK, US & Europe Distribution

Application: This Human Histone deacetylase-2 (HDAC2) ELISA Kit employs a two-site sandwich ELISA to quantitate HDAC2 in samples. An antibody specific for HDAC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHDAC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HDAC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HDAC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Histone deacetylase-2 microplate
• Human Histone deacetylase-2 standard
• Human Histone deacetylase-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Histone deacetylase-2 (HDAC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human HDAC2. No significant cross-reactivity or interference between Human HDAC2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Histone deacetylase 2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4) . This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events.YY1 is a mammalian zinc finger transcription factor that acts as both a positive and negative regulator of transcription at CCATNTT consensus DNA elements in promoters of cellular and viral genes.

Alternative Names: HDAC2; RPD3; YAF1; YY1-associated factor 1; transcriptional regulator homolog RPD3

Search name: HDAC2; RPD3; YAF1; YY1-associated factor 1; transcriptional regulator homolog RPD3

Tag: HDAC2