Human Histone H2B type 1-D (HIST1H2BD) ELISA Kit | KTE62387

Human Histone H2B type 1-D (HIST1H2BD) ELISA Kit | KTE62387 | Gentaur UK, US & Europe Distribution

Application: This Human Histone H2B type 1-D (HIST1H2BD) ELISA Kit employs a two-site sandwich ELISA to quantitate HIST1H2BD in samples. An antibody specific for HIST1H2BD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHIST1H2BD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST1H2BD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST1H2BD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Histone H2B type 1-D microplate
• Human Histone H2B type 1-D standard
• Human Histone H2B type 1-D detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Histone H2B type 1-D (HIST1H2BD) ELISA Kit has high sensitivity and excellent specificity for detection of Human HIST1H2BD. No significant cross-reactivity or interference between Human HIST1H2BD and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. Albig et al. (1991) identified a gene encoding a member of the H2B class of histones and designated it H2B.1B. Albig and Doenecke (1997) designated this gene H2B/b.By analysis of a YAC contig, Albig et al. (1997) mapped the H2B/b gene to chromosome 6p21.3, within a cluster of 35 histone genes.

Alternative Names: HIST1H2BD; H2B.1B; H2B/b; H2BFB; HIRIP2; MGC90432; dJ221C16.6; H2B histone family; member B; OTTHUMP00000016143; histone 1; H2bd

Search name: HIST1H2BD; H2B.1B; H2B/b; H2BFB; HIRIP2; MGC90432; dJ221C16.6; H2B histone family; member B; OTTHUMP00000016143; histone 1; H2bd

Tag: HIST1H2BD