Human Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L) ELISA Kit | KTE61989

Human Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L) ELISA Kit | KTE61989 | Gentaur UK, US & Europe Distribution

Application: This Human Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L) ELISA Kit employs a two-site sandwich ELISA to quantitate DOT1L in samples. An antibody specific for DOT1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOT1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOT1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOT1L bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Histone-lysine N-methyltransferase, H3 lysine-79 specific microplate
• Human Histone-lysine N-methyltransferase, H3 lysine-79 specific standard
• Human Histone-lysine N-methyltransferase, H3 lysine-79 specific detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L) ELISA Kit has high sensitivity and excellent specificity for detection of Human DOT1L. No significant cross-reactivity or interference between Human DOT1L and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DOT1L is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. DOT1L is a nucleosomal histone-3 (H3) -specific methyltransferase that requires the SAM and sequences between amino acids 351 and 472 for activity. Overexpression of DOT1L in human embryonic kidney cells significantly increased methylation of lys79 of H3, but had no effect on lys4 or lys9. Methylation of lys79, attributed to DOT1L, was regulated during the cell cycle in synchronized HeLa cells. The deduced 1, 537-amino acid protein has a calculated molecular mass of 165 kD and contains a SAM (sterile alpha motif) domain for protein-protein interaction. DOT1L does not, however, have the SET domain found in most histone lysine methyltransferases.

Alternative Names: DOT1L; DKFZp586P1823; DOT1; KIAA1814; KMT4; DOT1-like; histone H3 methyltransferase; histone methyltransferase DOT1L

Search name: DOT1L; DKFZp586P1823; DOT1; KIAA1814; KMT4; DOT1-like; histone H3 methyltransferase; histone methyltransferase DOT1L

Tag: DOT1L