Human HLA-B associated transcript 3 (BAT3) ELISA Kit | KTE62697

Human HLA-B associated transcript 3 (BAT3) ELISA Kit | KTE62697 | Gentaur UK, US & Europe Distribution

Application: This Human HLA-B associated transcript 3 (BAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate BAT3 in samples. An antibody specific for BAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human HLA-B associated transcript 3 microplate
• Human HLA-B associated transcript 3 standard
• Human HLA-B associated transcript 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human HLA-B associated transcript 3 (BAT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human BAT3. No significant cross-reactivity or interference between Human BAT3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: BAT3 contains an N-terminal ubiquitin-like domain, and both BAT2 and BAT3 are rich in proline and include short tracts of polyproline, polyglycine, and charged amino acids.The presence of additional genes was suggested by a large cluster of CpG islands. With cosmid probes, 5 distinct transcripts, including BAT3, were detected in RNA samples from a variety of cell lines, and the corresponding cDNA clones were isolated.BAT3 formed a complex with p300 (EP300), and an increased amount of BAT3 enhanced recruitment of p53 to p300 and facilitated subsequent p53 acetylation. In contrast, Bat3-depleted cells showed reduced p53-p300 complex formation and decreased p53 acetylation. Thymocytes from Bat3-deficient mice exhibited reduced p53-mediated induction of Puma and p21 and were resistant to DNA damage-induced apoptosis in vivo.

Alternative Names: BAT3; DAQB-195H10.3; BAG-6; BAG6; D6S52E; G3; HLA-B associated transcript-3; large proline-rich protein BAT3; scythe HLA-B associated transcript 3 (BAT3)

Search name: BAT3; DAQB-195H10.3; BAG-6; BAG6; D6S52E; G3; HLA-B associated transcript-3; large proline-rich protein BAT3; scythe HLA-B associated transcript 3 (BAT3)

Tag: BAT3