Human HMBS (Hydroxymethylbilane Synthase) ELISA Kit | ELK4111

Human HMBS (Hydroxymethylbilane Synthase) ELISA Kit | ELK4111 | Gentaur UK, US & Europe Distribution

Alternative Names: PBG-D; PBGD; UPS; Hydroxymethylbilane Synthase; Uroporphyrinogen I Synthase; Porphobilinogen deaminase; Pre-uroporphyrinogen synthase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.26 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylbilane Synthase (HMBS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hydroxymethylbilane Synthase (HMBS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxymethylbilane Synthase (HMBS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxymethylbilane Synthase (HMBS) in the samples is then determined by comparing the OD of the samples to the standard curve.