Human HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit | ELK4646

Human HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit | ELK4646 | Gentaur UK, US & Europe Distribution

Alternative Names: FJHN; TCF2; HNF1βeta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2, Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.047 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta (HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta (HNF1b) in the samples is then determined by comparing the OD of the samples to the standard curve.