Human Homovanillic acid (HVA) ELISA Kit | AE63032HU

Human Homovanillic acid (HVA) ELISA Kit | AE63032HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: HVA

Alternative Name: N/A

Application: ELISA

Range: 2.47-200 ng/mL

Sensitivity: 0.97 ng/mL

Intra-Assay: ≤5.3%

Inter-Assay: ≤9.4%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate HVA in samples. An antibody specific for HVA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHVA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HVA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HVA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Homovanillic acid (HOC6H3 (OCH3) CH2COOH; synonyms: 3-Methoxy-4-hydroxyphenyl acetic acid; HVA; 4-Hydroxy-3-methoxy-benzeneacetic acid; 4-Hydroxy-3-methoxyphenylacetic acid) is a major catecholamine metabolite. It is used as a reagent to detect oxidative enzymes, and is associated with dopamine levels in the brain.In psychiatry and neuroscience, brain and cerebrospinal fluid levels of HVA are measured as a marker of metabolic stress caused by 2-deoxy-D-glucose. HVA presence supports a diagnosis of neuroblastoma and malignant pheochromocytoma. An acid that is produced by the normal metabolism of dopamine and that may occur at an elevated level in urine in association with tumors of the adrenal gland. Its normal accumulation in a 24-hour collection urine sample is 15 mg.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .