Human HSD17b1 (17-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit | ELK4895

Human HSD17b1 (17-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit | ELK4895 | Gentaur UK, US & Europe Distribution

Alternative Names: EDH17B2; EDHB17; HSD17; SDR28C1; Estradiol 17-Beta-Dehydrogenase 1; Short Chain Dehydrogenase/Reductase Family 28CE, 1; 20 alpha-hydroxysteroid dehydrogenase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) in the samples is then determined by comparing the OD of the samples to the standard curve.