Human HSD17b3 (17-Beta-Hydroxysteroid Dehydrogenase Type 3) ELISA Kit | ELK3762

Human HSD17b3 (17-Beta-Hydroxysteroid Dehydrogenase Type 3) ELISA Kit | ELK3762 | Gentaur UK, US & Europe Distribution

Alternative Names: SDR12C2; EDH17B3; Short Chain Dehydrogenase/Reductase Family 12C, Member 2; Testosterone 17-Beta-Dehydrogenase 3; Testicular 17-beta-hydroxysteroid dehydrogenase

Species: Human

Assay Type: Sandwich

Sensitivity: 6.4 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) in the samples is then determined by comparing the OD of the samples to the standard curve.