Human HSP90α (Heat Shock Protein HSP 90-alpha) ELISA Kit | ELK9054

Human HSP90α (Heat Shock Protein HSP 90-alpha) ELISA Kit | ELK9054 | Gentaur UK, US & Europe Distribution

Alternative Names: FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|Heat shock protein HSP 90-alpha

Species: Human

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates

Assay Lenght: 3.5h

Research Area: Neuro science, Stress response

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein HSP 90-alpha (HSP90α) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein HSP 90-alpha (HSP90α) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein HSP 90-alpha (HSP90α), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein HSP 90-alpha (HSP90α) in the samples is then determined by comparing the OD of the samples to the standard curve.