Human HSPA1A (Heat Shock 70kDa Protein 1A) ELISA Kit | ELK1755

Human HSPA1A (Heat Shock 70kDa Protein 1A) ELISA Kit | ELK1755 | Gentaur UK, US & Europe Distribution

Alternative Names: HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

Species: Human

Assay Type: Sandwich

Sensitivity: 0.6 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Infection immunity;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1A (HSPA1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 1A (HSPA1A) in the samples is then determined by comparing the OD of the samples to the standard curve.