Human HSPG2 (Heparan Sulfate Proteoglycan 2) ELISA Kit | ELK2974

Human HSPG2 (Heparan Sulfate Proteoglycan 2) ELISA Kit | ELK2974 | Gentaur UK, US & Europe Distribution

Alternative Names: PLC; PRCAN; SJA; SJS; SJS1; Perlecan; Endorepellin; Schwartz-Jampel Syndrome 1; Basement membrane-specific heparan sulfate proteoglycan core protein

Species: Human

Assay Type: Sandwich

Sensitivity: 52 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate Proteoglycan 2 (HSPG2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparan Sulfate Proteoglycan 2 (HSPG2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate Proteoglycan 2 (HSPG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate Proteoglycan 2 (HSPG2) in the samples is then determined by comparing the OD of the samples to the standard curve.