Human HTN3 (Histatin 3) ELISA Kit | ELK4644

Human HTN3 (Histatin 3) ELISA Kit | ELK4644 | Gentaur UK, US & Europe Distribution

Alternative Names: HIS2; HTN2; HTN5; Hst; PB; Histidine-rich protein 3; Basic histidine-rich protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.56 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Saliva, tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histatin 3 (HTN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Histatin 3 (HTN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histatin 3 (HTN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histatin 3 (HTN3) in the samples is then determined by comparing the OD of the samples to the standard curve.