Human IER3 (Immediate Early Response 3) ELISA Kit | ELK5015

Human IER3 (Immediate Early Response 3) ELISA Kit | ELK5015 | Gentaur UK, US & Europe Distribution

Alternative Names: PRG1; DIF-2; DIF2; GLY96; IEX-1; IEX-1L; IEX1; Radiation-inducible immediate-early gene IEX-1; Differentiation-dependent gene 2 protein; PACAP-responsive gene 1 protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immediate Early Response 3 (IER3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immediate Early Response 3 (IER3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immediate Early Response 3 (IER3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immediate Early Response 3 (IER3) in the samples is then determined by comparing the OD of the samples to the standard curve.