Human IGF1 protein | PRP100153

Human IGF1 protein | PRP100153 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from mature form of human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) was expressed and purified.

Activity: 1. Measured by its ability to bind human IGFBP4-His (Cat:10967-H08H) in a functional ELISA. 2. Measured by its ability to bind mouse IGFBP4-His (Cat:50250-M08H) in a functional ELISA. 3. Measured by its ability to bind Cynomolgus IGFBP1-His (Cat:90085-C08H) in a functional ELISA. 4. Measured by its ability to bind human IGF1R (aa 954-1367) -GST (Cat:10164-H20B1) in a functional ELISA. 5. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.5-2 ng/mL. 6. Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 1-4 ng/mL.

protein Lenght: The recombinant mature form of human IGF1 consisting of 71 amino acids and has a calculated molecular mass of 7.8KDa.

Preparation: E. coli

Purity: > 95 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile 30% Acetonitrile, 0.1% TFA

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 7.8kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IGF1 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IGF1 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.50% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ) and possibly the β-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.

Alternative Names: IGF-1; IGF-I; IGF1A; IGFI; IGF‑I; MGF; Insulin-like growth factor I; IGF1

Search Names: IGF-1; IGF-I; IGF1A; IGFI; IGF‑I; MGF; Insulin-like growth factor I; IGF1

Tag: IGF1