Human IGFIIE (Pro-Insulin Like Growth Factor II E-Peptide) ELISA Kit | ELK4859

Human IGFIIE (Pro-Insulin Like Growth Factor II E-Peptide) ELISA Kit | ELK4859 | Gentaur UK, US & Europe Distribution

Alternative Names: Preptin; Proinsulin-Like Growth Factor-II

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 13.5 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Infection immunity;Hepatology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) in the samples is then determined by comparing the OD of the samples to the standard curve.