Human IgG Protein | AB1010

Human IgG Protein | AB1010-1000 | Proteins

Description:

Human IgG full length protein isolated from human serum. IgG is a monomeric immunoglobulin, with two heavy chains and two light chains. IgG can be found in blood and is the most abundant immunoglobulin, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses (IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%)) that can bind to many types of pathogens, protecting the body against them by complement activation, opsonization for phagocytosis and neutralisation of their toxins. This protein can be used in biochemical and cell biology experiments as a blocking agent, coating protein, standard, reference antigen in a variety of immune assays including Western and dot blotting, ELISA, immune diffusion and immunoelectrophoresis.

Storage conditions:

Store at -80 C for long-term storage.

Host:

Human

Protein Type:

Native

Applications:

SDS-PAGE

Purity:

>95% by SDS-PAGE

Concentration:

10 mg/ml

Buffer:

Phosphate buffer (0.1 M, pH 7.4) and 0.001% sodium azide

Form:

Human full length IgG protein supplied as a 1 ml (10 mg/ml) aliquot in phosphate buffer (0.1 M, pH 7.4) and 0.001% sodium azide. This protein was affinity purified from serum of four donors tested and found negative for Hepatitis B, Hepatitis C, HIV I and II antibodies using protein G.