Human IL-1β protein (pro form), His Tag | PRP100050

Human IL-1β protein (pro form), His Tag | PRP100050 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from pro form of human IL-1β (NP_000567.1) (Met 1-Ser 269) was fused with a polyhistidine tag at the N-terminus.

Activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human IL-1B at 10 μg/mL (100 μl/well) can bind human IL-1R1. The EC50 of human IL-1R1 is 0.198 μg/mL.

protein Lenght: The recombinant pro form of human IL-1β consisting of 280 amino acids and has a calculated molecular mass of 32.3 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.

Preparation: E. coli

Purity: > 95 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 32.3 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 beta protein (pro form), His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-1β protein (pro form), His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.34% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Interleukin-1β (IL-1β or IL-1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL-1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL-1 superfamily consists of three members, IL-1A (IL-1 α), IL-1B (IL-1β), and IL-1 receptor antagonist (IL-1Ra) . In clinical, it has been reported that Interleukin (IL) -1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL) -1 gene polymorphisms associated with high levels of IL-1β activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL-1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL-2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc) .

Alternative Names: IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B

Search Names: IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B

Tag: IL-1β