Human IL-1α protein | PRP100048

Human IL-1α protein | PRP100048 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from the processed form of human IL-1α (NP_000566.3) (Ser 113-Ala 271) was expressed and purified.

Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 0.2-1 ng/mL .

protein Lenght: The recombinant human IL-1α consists of 159 amino acids and has a predicted molecular mass of 18 kDa. As a result of glycosylation, the apparent molecular mass of rhIL-1α is approximately 22 kDa in SDS-PAGE under reducing conditions.

Preparation: HEK293 Cells

Purity: > 95 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 18 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 alpha protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-1α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.33% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: IL-1 α is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6 and TNF-α, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13 and IL-5, are synthesized from Th2 immune cells. IL-1 α is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 α stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.

Alternative Names: IL-1A; IL-1F1; IL1; IL1-ALPHA; IL1F1; Interleukin-1 alpha; IL1A

Search Names: IL-1A; IL-1F1; IL1; IL1-ALPHA; IL1F1; Interleukin-1 alpha; IL1A

Tag: IL-1α