Human IL-22 protein | PRP100308

Human IL-22 protein | PRP100308 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from human IL-22 (NP_065386.1) (Ala34-Ile179) was expressed with a N-terminal Met.

Activity: Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.

protein Lenght: The recombinant human IL-22 consists of 147 amino acids and predicts a molecular mass of 16.9 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.

Preparation: E. coli

Purity: > 95 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 8.0.

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 16.9 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL22 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-22 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.77% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: IL-22 is a member of a group of cytokines called the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26),   a class of potent mediators of cellular inflammatory responses. It shares use of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1) . IL-22 and IL-10 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.

Alternative Names: IL-21; IL-22; IL-D110; IL-TIF; ILTIF; TIFa; TIFIL-23; zcyto18; Interleukin-22; IL22

Search Names: IL-21; IL-22; IL-D110; IL-TIF; ILTIF; TIFa; TIFIL-23; zcyto18; Interleukin-22; IL22

Tag: IL-22